Acridine orange staining of virus infected host cells to monitor proliferation of viral infection
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Immunofluorescence and cytochemical studies of visna virus in cell culture.
Sequential morphological changes occurring in sheep choroid plexus cells infected with visna virus were studied by direct immunofluorescence, acridine orange, and hematoxylin and eosin staining methods. Specific immunofluorescence was first detected in the perinuclear cytoplasm of solitary cells 24 hr after infection. As the infection progressed, viral antigen appeared in an increasing number o...
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